Peptide synthesis catalyzed by native proteinase K in water-miscible organic solvents with low water content

V Čeřovský, K Martinek

文献索引：Cerovsky, Vaclav; Martinek, Karel Collection of Czechoslovak Chemical Communications, 1989 , vol. 54, # 7 p. 2027 - 2041

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被引用次数: 10

摘要

Abstract Reaction of Ac-Tyr-OEt with HBr. Gly-NH 2, catalyzed by free proteinase K in various water-miscible organic solvents in the presence of triethylamine and 5 vol.% of water, was studied. Some aliphatic alcohols and acetonitrile proved to be suitable solvents. The effect of water content (2%-20%) on the synthesis of Ac-Tyr-Gly-NH 2 was studied using acetonitrile as solvent. Lowering of the water content to 5% or 2% led to almost 100% ...