... For maintenance, cells were grown in 75 cm 2 T-flasks (25 ml culture volume) to a cell density of circa 1.5 × 10 6 cells/ml and passaged every other day into fresh prewarmed (37°C) culture medium (inoculum cell density 0.5 ... 2 Orive G, Maria Hernàndez R, Rodríguez Gascón A ...