Organic nanoparticles with ultrahigh quantum yield and aggregation-induced emission characteristics for cellular imaging and real-time two-photon lung vasculature imaging
Bin Liu, Maximilien Evrard, Xiaolei Cai, Guangxue Feng, Nikodem Tomczak, Lai Guan Ng, Jie Liu
Fluorescent organic nanoparticles based on small molecules have emerged as an attractive class of fluorescent agents for bioimaging in recent years. Herein, we report an orange light-emitting BTPEBD based organic nanoparticles (BTPEBD NPs) with a large Stokes shift (> 135 nm), ultrahigh quantum yield (> 90% in water) and aggregation-induced emission characteristics. Single nanoparticle analysis studied by wide field microscopy imaging further proves that BTPEBD NPs exhibit high brightness and good photostability. Both in-vitro and in-vivo experiments reveal that BTPEBD NPs are promising fluorescent agents for cellular imaging and real-time two-photon lung vasculature imaging.