Lipid A structure and immunoinhibitory effect of the marine bacterium Cobetia pacifica KMM 3879T
Flaviana Di Lorenzo; Angelo Palmigiano; Sami Albitar-Nehme; Mateusz Pallach; Maxim Kokoulin; Nadezhda Komandrova; Lyudmila Romanenko; Maria Lina Bernardini; Domenico Garozzo; Antonio Molinaro; Alba Silipo
The structural elucidation of lipopolysaccharides (LPSs) from Gram‐negative marine bacteria, in addition to the assessment of their immunological properties, is a fascinating and active research field, not only because it can give insight to aid understanding of adaptation phenomena occurring in the marine environment but also it opens new perspectives of design and development of novel immunoregulatory drugs. Here we report the structural characterization of the lipid A component of the LPS isolated from the marine bacterium Cobetia pacifica KMM 3879T, characterized by a family of species differing in the acylation pattern. The structure has been achieved by extensive chemical analyses and matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry. Moreover, cellular immunology studies on the LPS highlighted its low immunostimulatory impact and a very interesting and promising inhibitory activity of the toxic effects of Escherichia coli LPS.