The novel 7-propinyloxy-3-(p-propinyloxyphenyl)coumarin which has double terminal ethynly groups was synthesized for the first time. This coumarin has been successfully bounded covalently to single walled carbon nanotubes (SWCNTs) via the azide-alkyne Huisgen cycloaddition (Click) reaction for the preparation of a 3D hybrid material. Glassy carbon electrode was modified with the SWCNTs-coumarin 3D hybrid material for the determination of quercetin antioxidant. The proposed modified electrode is nearly 10-fold more sensitive than the bare electrode by applying the differential pulse stripping voltammetry (DPSV). In addition, the parameters of DPSV measurements such as accumulation time, accumulation potential and pH were optimized as 20 s, 200 mV and pH 2 Britton-Robinson buffer solutions in 1 mM quercetin, respectively. In the optimized conditions, the linear working ranging from 0.25 to 3 μM was determined. The detection and quantification limits of quercetin were found to be 20 nM and 66 nM for quercetin by DPSV on modified electrode, respectively. The DPSV procedure was also applied to test different brand tea real samples and quercetin was determined by a standard addition method with the recovery between 97 and 102.3.