Surface plasmon resonance imaging (SPRi) is a label-free, non-invasion detection method that allows real-time analysis for biomolecular interactions with high sensitivity and high throughput. Cellular analysis and detection is significant in many research areas, including healthcare, food safety, environmental and agricultural monitoring, as well as for the application of SPRi. This review summarizes the utilization of SPRi for cellular analysis and detection in the last decade and illustrates the boundless potential of the SPRi technique in these fields.