Investigating Inner-Sphere Reorganization via Secondary Kinetic Isotope Effects in the C− H Cleavage Reaction Catalyzed by Soybean Lipoxygenase: Tunneling in …

MP Meyer, JP Klinman

文献索引：Meyer, Matthew P.; Klinman, Judith P. Journal of the American Chemical Society, 2011 , vol. 133, # 3 p. 430 - 439

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被引用次数: 19

摘要

This work describes the application of NMR to the measurement of secondary deuterium (2° 2H) and carbon-13 (13C) kinetic isotope effects (KIEs) at positions 9− 13 within the substrate linoleic acid (LA) of soybean lipoxygenase-1. The KIEs have been measured using LA labeled with either protium (11, 11-h2-LA) or deuterium (11, 11-d2-LA) at the reactive C11 position, which has been previously shown to yield a primary deuterium isotope effect of ...