The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

MC Gutiérrez, A Sleegers, HD Simpson…

文献索引：Gutierrez, Maria C.; Sleegers, Arthur; Simpson, Helen D.; Alphand, Veronique; Furstoss, Roland Organic and Biomolecular Chemistry, 2003 , vol. 1, # 20 p. 3500 - 3506

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被引用次数: 45

摘要

The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer–Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether 1 as a fluorogenic substrate. When Baeyer–Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. ...