Combined chemical and enzymatic synthesis of sialyloligosaccharides and characterization by 500-MHz and proton and carbon-13 NMR spectroscopy

S Sabesan, JC Paulson

文献索引：Sabesan,S.; Paulson,J.C. Journal of the American Chemical Society, 1986 , vol. 108, p. 2068

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被引用次数: 182

摘要

Abstract: Sialyloligosaccharides that occur as terminal sequences in glycoproteins and glycolipids were synthesized by using combined chemical and enzymatic methodologies. Neutral oligosaccharides containing PDGal (1, 3) P~ GlcNAc (type l), gal (1, 4) P~ Glc (NAc)(type 2), and pDGal (1, 3)@~ GalNAc (type 3) sequences were sialylated enzymatically by using three purified mammalian sialyltransferases each of which uses ...