Abstract Lipase-catalyzed synthesis of esters of anthranilic acid was attempted by employing alcohols of carbon chainlength C 1–C 18 using Plackett-Burman experimental design. Of the alcohols employed, methanol, decanol, cetyl alcohol, and stearyl alcohols showed 99.9% significance. Esterification of anthranilic acid with methanol gave the highest yield at 45.6%. This study allows the selection of better alcohols for esterification of anthranilic acid.