Synthesis of 4-Hydroxyisoleucine by the Aldolase–Transaminase Coupling Reaction and Basic Characterization of the Aldolase from Arthrobacter simplex AKU 626

J Ogawa, H Yamanaka, J Mano, Y Doi…

文献索引：Bioscience, Biotechnology and Biochemistry, , vol. 71, # 7 p. 1607 - 1615

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被引用次数: 19

摘要

... Biochem., 71 (7), 1607–1615, 2007 ... The dia- lyzed enzyme solution was applied to a Mono Q HR 10/ 10 column (Amersham Pharmacia Biotech, Uppsala, Sweden) equilibrated with buffer A. After it was washed with buffer A, the enzyme was eluted with a linear gradient of 1–0 ...