Highly Efficient Chemoenzymatic Synthesis of Methyl (R)??o??Chloromandelate, a Key Intermediate for Clopidogrel, via Asymmetric Reduction with Recombinant …
Abstract Methyl (R)-o-chloromandelate [(R)-1], which is an intermediate for a platelet aggregation inhibitor named clopidogrel, was obtained in> 99% ee by the asymmetric reduction of methyl o-chlorobenzoylformate (2) with recombinant Escherichia coli overproducing a versatile carbonyl reductase. A remarkable temperature effect on productivity was observed in the whole-cell reduction of 2, and the optimum productivity as ...
