Microorganisms that produce ascorbic acid-2-phosphate (AsA2P) from ascorbic acid (AsA) and ATP were screened from our culture collection. Of 715 strains, three microorganisms, Pseudomonas azotocolligans KY4661, P. paucimobilis KY4084, and an unidentified bacterium KY3132 were selected as AsA2P-producers. When using 50mg (wet weight) of P. azotocolligans cells per ml as the enzyme source, 3.7 mM AsA2P were produced for 23 hr, ...