Collection of Czechoslovak chemical communications

Synthesis of Guanosine-3'-(5-bromo-4-chloroindol-3-yl) p-hosphate (G-3'-BCIP)

A Haikal

文献索引：Haikal, Abdelfattah Collection of Czechoslovak Chemical Communications, 1996 , vol. 61, # 3 p. 427 - 431

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被引用次数: 1

摘要

Chromogenic substrates are routinely used for spectroscopic analysis of enzyme activity1,2. The 5-bromo-4-chloroindol-3-yl group may be a particularly useful group in identifying ribonuclease activity. Specific ribonucleases should cleave the bond between the phos- phate and the chromophore. The oxidation of the resulting 5-bromo-4-chloroindol-3-ol to a dimeric product results3 in an important absorbance increase between 600 and 700 nm. For this reason a ...