(21) Ridder, A.; Sandford, K. K.; Evans, V. J. J. Natl. Cancer Inst. 1972,49, 1705. (22) Scholl, S. M.; Huff, KK; Lippman, ME Endocrinology 1983,113, 611. ... At the fourth day, media were changed. Three days later, cells were labeled with 1 pCi [3H]thymidine/well for 2 h. Cells were washed with cold PBS and harvested in PBS containing 0.02% EDTA. After centrifugation, the cell pellet was resuspended in 1 mL of PBS and divided in two equal parts. One part was ...