Tetrahedron

Synthesis and Enzymatic Digestion of an RNA nonamer in both enantiomeric forms

E Moyroud, E Biala, P Strazewski

文献索引：Moyroud, Elisabeth; Biala, Ewa; Strazewski, Peter Tetrahedron, 2000 , vol. 56, # 11 p. 1475 - 1484

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被引用次数: 25

摘要

The d-and l-RNA nonamers of the sequence r (GCUUCGGC) T have been synthesised for X- ray crystallographic purposes. In vitro digestion of the unnatural optical antipode by snake venom phosphodiesterase I takes place at an approximately 1800-fold slower rate than that of the natural d-nonamer. The digestion experiments showed—to our knowledge for the first time—that l-RNA can indeed be cleaved enzymatically when phosphodiesterase I from ...