Biochemistry

Essential role of the donor acyl carrier protein in stereoselective chain translocation to a fully reducing module of the nanchangmycin polyketide synthase

X Guo, T Liu, Z Deng, DE Cane

文献索引：Guo, Xun; Liu, Tiangang; Deng, Zixin; Cane, David E. Biochemistry, 2012 , vol. 51, # 4 p. 879 - 887

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被引用次数: 9

摘要

Incubation of recombinant module 2 of the polyether nanchangmycin synthase (NANS), carrying an appended thioesterase domain, with the ACP-bound substrate (2 RS)-2-methyl- 3-ketobutyryl-NANS_ACP1 (2-ACP1) and methylmalonyl-CoA in the presence of NADPH gave diastereomerically pure (2 S, 4 R)-2, 4-dimethyl-5-ketohexanoic acid (4a). These results contrast with the previously reported weak discrimination by NANS module 2+ TE ...