A new class of Spiegelmers containing 2′-fluoro-nucleotides

…, G Bahrenberg, C Gillen, S Klussmann…

文献索引：Helmling; Moyroud; Schroeder; Roehl; Kleinjung; Stark; Bahrenberg; Gillen; Klussmann; Vonhoff Nucleosides, Nucleotides and Nucleic Acids, 2003 , vol. 22, # 5-8 p. 1035 - 1038

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被引用次数: 8

摘要

Spiegelmers are metabolically stable enantiomeric nucleic acid ligands with high affinity and specificity for a given target. Employing a modified SELEX process (Systematic Evolution of Ligands by Exponential enrichment) with a neuropeptide target consisting of D-amino acids and modified nucleoside triphosphates (2′-fluoro-dUTP and 2′-fluoro-dCTP), we identified 6 different sequences containing 2′-fluoro-dU and 2′-fluoro-dC that specifically bind the enantiomeric target. ...