Identification of residues critical for catalysis in a class C β??lactamase by combinatorial scanning mutagenesis

SD Goldberg, W Iannuccilli, T Nguyen, J Ju…

文献索引：Nishizawa, Masako; Yabusaki, Yoshiyasu; Kanaoka, Masaharu Bioscience, Biotechnology and Biochemistry, 2011 , vol. 75, # 1 p. 89 - 94

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被引用次数: 35

摘要

... The MegaBACE 1000 capillary sequencer was purchased from Amersham Biosciences, as were the ECL-Plus chemiluminescence ... min, and finally 30 min at 68°C. Cycle sequencing (20-μL final reaction volume) was performed ... C for 20 sec, 54°C for 20 sec, and 60° C for 1 min. ...