... 0.07 M –1 s –1 , respectively) and produce quantitative yield of ligation in organic solvents or ... reactions, eg, CuAAC and Diels–Alder,(11a, 12a-12d) SPAAC and SPANC,(15, 13a, 13b ... isolated from the supernatant via spin filtration and reconstituted to the original volume in PBS. ...