前往化源商城

Journal of Pharmaceutical Sciences 2015-02-01

Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces.

Sebastian J Kapp, Iben Larsson, Marco Van De Weert, Marité Cárdenas, Lene Jorgensen

文献索引:J. Pharm. Sci. 104(2) , 593-601, (2015)

全文:HTML全文

摘要

Two monoclonal antibodies from the IgG subclasses one and two were compared in their adsorption behavior with hydrophobic surfaces upon dilution to 10 mg/mL with 0.9% NaCl. These conditions simulate handling of the compounds at hospital pharmacies and surfaces encountered after preparation, such as infusion bags and i.v. lines. Total internal reflection fluorescence and quartz crystal microbalance with dissipation monitoring were used to follow and quantify this. Furthermore, the influence of the nonionic surfactant polysorbate 80 (PS80) on the adsorption process of these two antibodies was investigated. Despite belonging to two different IgG subclasses, both antibodies displayed comparable adsorption behavior. Both antibodies readily adsorbed in the absence of PS80, whereas adsorption was reduced in the presence of 30 mg/L surfactant. The sequence of exposure of the surfactant and protein to the surface was found to have a major influence on the extent of protein adsorption. Although only a fraction of adsorbed protein could be removed by rinsing with 30 mg/L surfactant solution, adsorption was entirely prevented when surfaces were pre-exposed to PS80.© 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

相关化合物

结构式 名称/CAS号 全部文献
硫酸 结构式 硫酸
CAS:7664-93-9
盐酸 结构式 盐酸
CAS:7647-01-0
氯化钠 结构式 氯化钠
CAS:7647-14-5
过氧化氢 结构式 过氧化氢
CAS:7722-84-1
氯化钠-35cl 结构式 氯化钠-35cl
CAS:20510-55-8
氨 结构式
CAS:7664-41-7
AMMONIA (14N) 结构式 AMMONIA (14N)
CAS:1026405-88-8
氯化氢甲醇溶液 结构式 氯化氢甲醇溶液
CAS:132228-87-6
丙硫醇 结构式 丙硫醇
CAS:107-03-9