前往化源商城

Journal of chromatography. A 2014-08-22

1.9 μm superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules.

Yi Min, Bo Jiang, Ci Wu, Simin Xia, Xiaodan Zhang, Zhen Liang, Lihua Zhang, Yukui Zhang

文献索引:J. Chromatogr. A. 1356 , 148-56, (2014)

全文:HTML全文

摘要

In this work, 1.9 μm reversed-phase packing materials with superficially porous structure were prepared to achieve the rapid and high efficient separation of peptides and proteins. The silica particles were synthesized via three steps, nonporous silica particle preparation by a modified seeded growth method, mesoporous shell formation by a one pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. By such a method, 1.9 μm superficially porous materials with 0.18 μm shell thickness and tailored pore diameter (10 nm, 15 nm) were obtained. After pore enlargement, the formerly dense arrays of mesoporous structure changed, the radially oriented pores dominated the superficially porous structure. The chromatographic performance of such particles was investigated after C18 derivatization. For packing materials with 1.9 μm diameter and 10 nm pore size, the column efficiency could reach 211,300 plates per m for naphthalene. To achieve the high resolution separation of peptides and proteins, particles with pore diameter of 15 nm were tailored, by which the baseline separation of 5 peptides and 5 intact proteins could be respectively achieved within 1 min, demonstrating the superiority in the high efficiency and high throughput analysis of biomolecules. Furthermore, BSA digests were well separated with peak capacity of 120 in 30 min on a 15 cm-long column. Finally, we compared our columns with a 1.7 μm Kinetex C18 column under the same conditions, our particles with 10nm pore size demonstrated similar performance for separation of the large intact proteins. Moreover, the particles with 15 nm pore size showed more symmetrical peaks for the separation of large proteins (BSA, OVA and IgG) and provided rapid separation of protein extracts from Escherichia coli in 5 min. All these results indicated that the synthesized 1.9 μm superficially porous silica packing materials would be promising in the ultra-fast and high-resolution separation of biomolecules.Copyright © 2014 Elsevier B.V. All rights reserved.

相关化合物

结构式 名称/CAS号 全部文献
乙腈 结构式 乙腈
CAS:75-05-8
氨水 结构式 氨水
CAS:1336-21-6
氟化铵 结构式 氟化铵
CAS:12125-01-8
戊基苯 结构式 戊基苯
CAS:538-68-1
三氟乙酸(TFA) 结构式 三氟乙酸(TFA)
CAS:76-05-1
单分散二氧化硅微球 结构式 单分散二氧化硅微球
CAS:7631-86-9
十六烷基三甲基氯化铵 结构式 十六烷基三甲基氯化铵
CAS:112-02-7
十六烷基三甲基溴化铵 结构式 十六烷基三甲基溴化铵
CAS:57-09-0
正十三烷 结构式 正十三烷
CAS:629-50-5
甲苯 结构式 甲苯
CAS:108-88-3