Applied Microbiology and Biotechnology 2008-11-01

Recovery of choline oxidase activity by in vitro recombination of individual segments.

Birgit Heinze, Nina Hoven, Timothy O'Connell, Karl-Heinz Maurer, Sebastian Bartsch, Uwe T Bornscheuer

文献索引：Appl. Microbiol. Biotechnol. 81(2) , 275-82, (2008)

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摘要

Initial attempts to express a choline oxidase from Arthrobacter pascens (APChO-syn) in Escherichia coli starting from a synthetic gene only led to inactive protein. However, activity was regained by the systematic exchange of individual segments of the gene with segments from a choline oxidase-encoding gene from Arthrobacter globiformis yielding a functional chimeric enzyme. Next, a sequence alignment of the exchanged segment with other choline oxidases revealed a mutation in the APChO-syn, showing that residue 200 was a threonine instead of an asparagine, which is, thus, crucial for confering enzyme activity and, hence, provides an explanation for the initial lack of activity. The active recombinant APChO-syn-T200N variant was biochemically characterized showing an optimum at pH 8.0 and at 37 degrees C. Furthermore, the substrate specificity was examined using N,N-dimethylethanolamine, N-methylethanolamine and 3,3-dimethyl-1-butanol.