An in vitro experimental model has been developed for erosive lesion formation in dental enamel which can mimic in vivo conditions at the tooth surface. The features of this model provide a very reproducible method of producing erosive lesions in several teeth under identical conditions which can be varied to allow examination of a number of different parameters. A replica impression technique has also been developed for subsequent non-destructive microscopic examination of the lesions and comparison with in vivo lesions.