Samples of saliva were collected from subjects with dentures. These samples were collected both before the dentures were replaced and 1 week after the subjects had received their new dentures. Dibutylphthalate and phenyl benzoate were detected in the saliva samples with a gas-chromatography and a gas-chromatography/mass-spectrometry technique. We also quantified the dibutylphthalate in the saliva. In addition, in an in vitro study, we identified biphenyl leached from heat-cured denture base polymer plates. Our study suggests that subjects with dentures have higher contents of the above organic substances in saliva than subjects without dentures and that organic additives leach from new heat-cured dentures.
