Journal of Inorganic Biochemistry 2011-05-01

Metal complexes as artificial proteases in proteomics: a palladium(II) complex cleaves various proteins in solutions containing detergents.

Frank Miskevich, Amy Davis, Porntip Leeprapaiwong, Virginia Giganti, Nenad M Kostić, Laurence A Angel

文献索引：J. Inorg. Biochem. 105(5) , 675-83, (2011)

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摘要

Most popular agents for site-specific protein cleavage are proteolytic enzymes. Because they become denatured and inactivated by detergents, enzymes are inconvenient for proteomic analysis of hydrophobic proteins which require detergents as solubilizing agents. We used cis-[Pd(en)(H(2)O)(2)](2+) (in which en represents ethylenediamine) as an artificial protease to effect cleavage of three bovine proteins, namely ubiquitin, β-casein, and serum albumin, in separate experiments. Cleavage took place in aqueous solutions containing 1.0%wt./vol. of either 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) or Zwittergent 3-14 at 2.5