A novel bonded stationary phase, tetradecylamine bonded stationary phase (TABP), for reversed-phase HPLC was prepared by bonding 1-tetradecylamine to YWG-80 silica gel through 3-glycidoxypropyltrimethoxysilane. Hydrophobicity, selectivity and silanophilic activity of TABP were evaluated by using aromatic compounds as analytes and methanol-water as binary mobile phase. The orgainc components including acidic, basic and neutral aromatic analytes could be separated satisfactorily with excellent selectivity and peak shape. The relative retention alpha for ethylbenzene-toluene was found to be 1.66 and the asymmetry factors of basic aniline, p-toluidine and N,N-dimethylaniline were found to be 1.26, 1.21 and 1.10, respectively, with the V(methanol):V(water) = 55:45 mobile phase. Aniline was eluted before phenol due to the suppress of the ion exchange activity of residual silanols by the internal masking interaction.
