Bioresource Technology 2012-06-01

BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae.

T Govender, L Ramanna, I Rawat, F Bux

文献索引：Bioresour. Technol. 114 , 507-511, (2012)

摘要

In order to develop feasible production processes for microalgal biodiesel, the isolation of high neutral lipid producing microalgae is crucial. Since the established Nile Red (NR) method for detection of intracellular lipids has been successful only for some microalgae, a more broadly applicable detection method would be desirable. Therefore, BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for detection of intracellular lipids in Chlorella vulgaris, Dunaliella primolecta and Chaetoceros calcitrans. An optimum concentration of 0.067 μg ml(-1) was determined for lipid staining in the microalgae. Compared to NR, BODIPY 505/515 was more effective in staining microalgae and showed resistance to photobleaching, maintaining its fluorescence longer than 30 min.Copyright © 2012 Elsevier Ltd. All rights reserved.

结构式	名称/CAS号	分子式	全部文献
	2,8-二乙基-5,5-二氟-1,3,7,9-四甲基-10-苯基-5H-4l4,5l4-二吡咯并[1,2-c:2',1'-f][1 ,3,2]二氮杂环己硼烷 CAS:189264-25-3	C₂₃H₂₇BF₂N₂
	5,5-difluoro-1,3,7,9-tetramethyl-10-phenyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide CAS:194235-40-0	C₁₉H₁₉BF₂N₂
	4,4-二氟-1,3,5,7-四甲基-4-硼-3A,4A-二氮杂-S-茚烯 CAS:21658-70-8	C₁₃H₁₅BF₂N₂

BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae.

摘要

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