Biochemical and Biophysical Research Communications 1994-02-28

Alcoholysis of epsilon-decalactone with polyethylene glycol-modified lipase in 1,1,1-trichloroethane.

M Furukawa, Y Kodera, T Uemura, M Hiroto, A Matsushima, H Kuno, H Matsushita, Y Inada

文献索引：Biochem. Biophys. Res. Commun. 199(1) , 41-5, (1994)

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摘要

Lipase from Pseudomonas cepacia was modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine, activated PEG2, to form PEG-lipase. The PEG-lipase is soluble and active in organic solvents. It catalyzes alcoholysis of racemic epsilon-decalactone with ethanol in 1,1,1-trichloroethane to form (R)-hydroxydecanoic acid ethyl ester. No alcoholysis of (S)-decalactone takes place. These results were discussed in relation to carbon number of n-alcohol, optimum temperature and comparison with modified and non-modified lipases.