Journal of Immunological Methods 1983-10-14

A simple and efficient method for the production of human gamma interferon.

I A Braude

文献索引：J. Immunol. Methods 63(2) , 237-46, (1983)

全文：HTML全文

摘要

A method for the production of human gamma interferon (HuIFN-gamma), which is both simple and efficient, has been developed. The 2 co-inducers, A-23187 and mezerein, stimulate human peripheral blood lymphocytes to produce high levels of HuIFN-gamma in both stationary cultures and spinner cultures. The compound 2,2-dimethyl-6,6,7,7,8,8,8-heptafluoro-3,5-octanedione (FOD) has also proven to be a useful co-inducer. The production of HuIFN-gamma appears to occur in at least 2 phases.