Enzyme and Microbial Technology 2012-12-10

Synthesis and characterization of ampelopsin glucosides using dextransucrase from Leuconostoc mesenteroides B-1299CB4: glucosylation enhancing physicochemical properties.

Hye-Jin Woo, Hee-Kyoung Kang, Thi Thanh Hanh Nguyen, Go-Eun Kim, Young-Min Kim, Jun-Seong Park, Duwoon Kim, Jaeho Cha, Young-Hwan Moon, Seung-Hee Nam, Yong-mei Xia, Atsuo Kimura, Doman Kim

文献索引：Enzyme Microb. Technol. 51(6-7) , 311-8, (2012)

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摘要

Novel ampelopsin glucosides (AMPLS-Gs) were enzymatically synthesized and purified using a Sephadex LH-20 column. Each structure of the purified AMPLS-Gs was determined by nuclear magnetic resonance, and the ionic product of AMPLS-G1 was observed at m/z 505 (C₂₁H₂₂O₁₃·Na)⁺ using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. AMPLS-G1 was identified as ampelopsin-4'-O-α-D-glucopyranoside. The optimum condition for AMPLS-G1, determined using response surface methodology, was 70 mM ampelopsin, 150 mM sucrose, and 1 U/mL dextransucrase, which resulted in an AMPLS-G1 yield of 34 g/L. The purified AMPLS-G1 displayed 89-fold increased water solubility and 14.5-fold browning resistance compared to those of AMPLS and competitive inhibition against tyrosinase with a K(i) value of 40.16 μM. This value was smaller than that of AMPLS (K(i)=62.56 μM) and much smaller than that of β-arbutin (K(i)=514.84 μM), a commercial active ingredient of whitening cosmetics. These results indicate the potential of AMPLS and AMPLS-G1 as superior ingredients for functional cosmetics.Copyright © 2012 Elsevier Inc. All rights reserved.