Biochimica et Biophysica Acta 2014-01-01

Extracellular metabolism-dependent uptake of lysolipids through cultured monolayer of differentiated Caco-2 cells.

Maiko Inaba, Kaeko Murota, Miki Nikawadori, Erika Kishino, Risa Matusda, Mika Takagi, Takeshi Ohkubo, Tamotsu Tanaka, Junji Terao, Akira Tokumura

文献索引：Biochim. Biophys. Acta 1841(1) , 121-31, (2014)

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摘要

Glycerophospholipids are known to be hydrolyzed in the intestinal lumen into free fatty acids and lysophospholipids that are then absorbed by the intestinal epithelial cells. A monolayer of enterocyte-differentiated Caco-2 cell is often used to assess the intestinal bioavailability of nutrients. In this study, we examined how differentiated Caco-2 cells process lysoglycerolipids such as lysophosphatidylcholine (LPC). Our findings were twofold. (1) Caco-2 cells secreted both a lysophospholipase A-like enzyme and a glycerophosphocholine-phosphodiesterase enzyme into the apical, but not basolateral, lumen, suggesting that food-derived LPC is converted to a free fatty acid, sn-glycerol-3-phosphate, and choline through two sequential enzymatic reactions in humans. The release of the latter enzyme was differentiation-dependent. (2) Fatty acid-releasing activities toward exogenous fluorescent LPC, lysophosphatidic acid and monoacylglycerol were shown to be higher on the apical membranes of Caco-2 cells than on the basolateral membranes. These results suggest that human intestinal epithelial cells metabolize lysoglycerolipids by two distinct mechanisms involving secreted or apical-selective expression of metabolic enzymes. © 2013.