Biotechnology Progress 1990-01-01

Effect of inclusion body production on culture turbidity and cell dry weight in growing bacterial cultures.

S O Hwang, R S Feldberg

文献索引：Biotechnol. Prog. 6(1) , 48-50, (1990)

全文：HTML全文

摘要

An approach to the optimization of product yield in an inducible inclusion body-producing system is presented. Following induction by indoleacrylic acid (IAA) of a trpLE-HIVgp41 fusion protein, we found a large increase in culture turbidity and single-cell dry weight. After an initial transition phase, new and constant values for specific growth rate, single-cell light turbidity, and single cell dry weight were achieved, allowing for the determination of optimal conditions for product formation.