FEMS Microbiology Letters 2011-10-01

Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

Ying Hou, Jian Tao, Wenjing Shen, Juan Liu, Jingquan Li, Yongfeng Li, Hui Cao, Zhongli Cui

文献索引：FEMS Microbiol. Lett. 323(2) , 196-203, (2011)

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摘要

An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. This strain converted FE by cleavage of the ester bond, but could not further degrade FA. Strain T1 could also efficiently degrade haloxyfop-R-methyl, quizalofop-p-ethyl, cyhalofop-butyl and clodinafop-propargyl. FE hydrolase capable of hydrolysing FE to FA was found in the cell-free extract of strain T1 by zymogram analysis. A novel gene feh encoding FE hydrolase was cloned by shotgun library construction and successfully expressed in Escherichia coli.© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.