Nucleic Acids Research 1993-11-11

Synthesis of a carboxamide linked T*T dimer and its incorporation in oligonucleotides.

A Chur, B Holst, O Dahl, P Valentin-Hansen, E B Pedersen

文献索引：Nucleic Acids Res. 21 , 5179, (1993)

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摘要

The condensation of 5'-O-protected 3'-O-(2-aminoethyl)thymidine with 1,2-dideoxy-1-thyminyl-beta-D-erythro-pentofuranuronic acid gives a T*T dimer with * representing a 3'-OCH2CH2NHC(O)-4' linkage connecting the two pentofuranosyl moieties. The incorporation of this dimer in oligonucleotide sequences show only moderately lowered Tm values when hybridized with a complementary DNA relative to the unmodified DNA duplex. Consistently, no looped-out or bubble-type structure could be detected in DNA duplexes with an internal T*T module. Moreover, the 5-atom carboxamide linker causes complete stop on DNA polymerization and on exonuclease III degradation.