The synthesis of VI-A containing [''C] C0, H (specific activity of 2.82 X same manner with the use of isotopically labeled KCN. Bacterial Studies. A stock culture of E. coli 9723 (obtd from American Type Culture Collection, Rockville, Md.) was maintained on nutrient agar slants by conventional bacteriological practices. For inoculation of the assays, cells were grown for 15- 17 hr at 37" in 10 ml of the salts-glucose medium described below. An aseptic transfer (0.1 ...
