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Synthesis and Characterization of photoactivatable doxycycline analogues bearing two-photon sensitive photoremovable groups suitable for light induced gene expression
10.1002/cbic.201700628 2018-01-17 We report herein the synthesis and the photolytic properties of EANBP and PEG7-DEACM caged 9-aminodoxycycline. 9-Aminodoxycycline is a tetracycline analogue able to activate transcription via the inducible TetOn transgene expression system and can be regiosel... |
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Exploring PTDH–P450BM3 Variants for the Synthesis of Drug Metabolites
10.1002/cbic.201700470 2018-01-17 Abstract The conversion of a series of pharmaceutical compounds was examined with three variants of cytochrome P450BM3 fused to phosphite dehydrogenase (PTDH) to enable cofactor recycling. Conditions for enzyme production were optimized, and the purified PTDH... |
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Control of protein activity and gene expression by cyclofen-OH uncaging
10.1002/cbic.201700630 2018-01-16 The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activable protein and the protein of interest to control the activity of the later, it i... |
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The Fourth Wave of Biocatalysis Emerges—The 13 th International Symposium on Biocatalysis and Biotransformations
10.1002/cbic.201700687 2018-01-16 Ride the wave! Biocatalysis uses nature's catalysts, enzymes and whole cell systems, for synthetic purposes. In a biotransformation, the biocatalyst transforms a well-defined substrate to the desired product, in contrast to the fermentation process, which pro... |
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Enzymes as Enhancers for the Biodegradation of Synthetic Polymers in Wastewater
10.1002/cbic.201700364 2018-01-16 Abstract Synthetic polyesters are today the second-largest class of ingredients in household products and are entering wastewater treatment plants (WWTPs) after product utilization. One approach to improve polymer biodegradation in wastewater would be to comp... |
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Efficient catalytic oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by magnetic laccase catalyst
10.1002/cbic.201800008 2018-01-15 2,5-Furandicarboxylic acid (FDCA) is a biobased platform chemical for the production of polyesters. The magnetic laccase catalyst with TEMPO as the mediator has the remarkable capability of oxidizing HMF to 2,5-furandicarboxylic acid (FDCA). Under optimal rea... |
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Investigating the accessibility of nucleosome by duocarmycin B2, Fe(II) peplomycin and MNase using capillary electrophoresis
10.1002/cbic.201700643 2018-01-15 Capillary electrophoresis coupled with DNA 5 Texas Red labeling was used to investigate the ability of duocarmycin B2, Fe(II) peplomycin, and MNase to access the nucleosome. Distinct accessibility patterns of these species to the nucleosome were observed. Duo... |
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Epitope-Resolved Detection of Peanut-Specific IgE Antibodies by Surface Plasmon Resonance Imaging
10.1002/cbic.201700694 2018-01-15 The front cover picture shows the selective binding of IgE-coated magnetic beads onto allergen-functionalized array spots. Binding is detected by surface plasmon resonance imaging (SPRi), as depicted with the reflected red light below the array. Anti-IgE anti... |
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Biocatalytic Synthesis of Nitriles through Dehydration of Aldoximes: The Substrate Scope of Aldoxime Dehydratases
10.1002/cbic.201700571 2018-01-14 Nitriles belong to the product classes, which are mostly needed and produced by the chemical industry, playing a major role in various industry segments ranging from high-volume low-price sectors such as polymers to low-volume high-price sectors such as the c... |
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Ketonization of proline residues in the peptide chains of actinomycins by a 4-Oxoproline synthase
10.1002/cbic.201700666 2018-01-12 X-type actinomycins (Acms) contain 4-hydroxyproline (Acm X0) or 4-oxoproline (Acm X2) in their ß-pentapeptide lactone rings whereas their α-ring contains proline. We demonstrate that these Acms are formed through asymmetric condensation of Acm half molecules ... |