7-羟基-10-氧化物-3H-吩恶嗪-3-酮

7-羟基-10-氧化物-3H-吩恶嗪-3-酮用途

重氮唑啉(重氮间苯二酚)是一种水溶性、无毒、稳定、膜透性的蓝色非荧光染料(微弱荧光)。Resazurin用作氧化还原指示剂,可在活细胞中还原为粉红色、高荧光Resorufin(Ex=530-560 nm,Em=590 nm)。瑞嗪可用于检测细胞(人、植物和动物、细菌和真菌)的活性、毒性、增殖、迁移和侵袭[1][2]。

7-羟基-10-氧化物-3H-吩恶嗪-3-酮名称

[ CAS 号 ]:
550-82-3

[ 中文名 ]:
7-羟基-10-氧化物-3H-吩恶嗪-3-酮

[ 英文名 ]:
3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide

[英文别名 ]:

3H-Phenoxazin-3-one,7-hydroxy-,10-oxide
azoresorcin
7-Hydroxy-10-oxy-phenoxazin-3-on
Diazoresorcinal
7-hydroxy-3h-fenoxazin-3-on-10-oxid
7-hydroxy-3h-phenoxazin-3-on10-oxide
PHENOXYAZIN-3-ONE,7-HYDROXY-,10-OXIDE
Resazurin tablets
7-HYDROXY-10-OXY-PHENOXAZIN-3-ONE
7-hydroxy-3h-phenoxazin-3-one10-oxide

7-羟基-10-氧化物-3H-吩恶嗪-3-酮生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

指南(以下是我们推荐的方案。该方案仅提供指南,应根据您的具体需要进行修改)。细胞存活率[1][2]：1.在37°C水浴中解冻瑞嗪溶液。2.将细胞放在96孔板上,用PBS清洗(避光)。3.取下PBS洗涤液,然后添加500μL(1µg/mL)瑞赛灵溶液。4.将平板放入培养箱中30分钟(培养时间取决于细胞类型和细胞数量)。5.使用微孔板阅读器测量Resorufin荧光(Ex=530-560 nm,Em=590 nm)。

[参考文献]

[1]. Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15.

[2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60.

7-羟基-10-氧化物-3H-吩恶嗪-3-酮物理化学性质

[ 密度 ]:
1.57g/cm3

[ 沸点 ]:
477.2ºC at 760mmHg

[ 分子式 ]:
C₁₂H₆NNaO₄

[ 分子量 ]:
251.17000

[ 闪点 ]:
242.4ºC

[ 精确质量 ]:
251.01900

[ PSA ]:
78.73000

[ LogP ]:
2.47010

[ 折射率 ]:
1.74

7-羟基-10-氧化物-3H-吩恶嗪-3-酮毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SP7700000

CHEMICAL NAME :: 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide

CAS REGISTRY NUMBER :: 550-82-3

BEILSTEIN REFERENCE NO. :: 0221382

LAST UPDATED :: 199701

DATA ITEMS CITED :: 4

MOLECULAR FORMULA :: C12-H7-N-O4

MOLECULAR WEIGHT :: 229.20

WISWESSER LINE NOTATION :: T C666 BN FV IOJ BO LQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 500 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: NCNSA6 National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,24,1953

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 179 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 44,225,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84815 No. of Facilities: 303 (estimated) No. of Industries: 1 No. of Occupations: 7 No. of Employees: 3485 (estimated) No. of Female Employees: 2576 (estimated)

7-羟基-10-氧化物-3H-吩恶嗪-3-酮安全信息

[ 海关编码 ]:
2934999090

7-羟基-10-氧化物-3H-吩恶嗪-3-酮海关

[ 海关编码 ]: 2934999090

[ 中文概述 ]:
2934999090. 其他杂环化合物. 增值税率:17.0%. 退税率:13.0%. 监管条件:无. 最惠国关税:6.5%. 普通关税:20.0%

[ 申报要素 ]: 品名, 成分含量, 用途

[ Summary ]:
2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%