108964-32-5

• 常用中文名：荧光钙探针 Fura 2-AM
• 常用英文名：Fura-2, AM
• CAS号：108964-32-5
• 分子式：C₄₄H₄₇N₃O₂₄
• 分子量：1001.850
• 相关类别： 染料试剂
• 发布时间：2018-08-17 00:41:27
• 更新时间：2024-01-08 10:58:35
• Fura-2 AM 是一种比率荧光 Ca2+ 指示剂。

名称

• 中文名: 荧光钙探针 Fura 2-AM
• 英文名: acetyloxymethyl 2-[5-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-4-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate
• 中文别名: AM荧光探针; 钙离子荧光探针Fura2-AM; 荧光钙探针Fura2-AM
• 英文别名: Fura 2am; Fura-2 pentakis(acetoxymethyl) ester; MFCD00036976; Acetoxymethyl 2-(6-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-[2-(2-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-methylphenoxy)ethoxy]-1-benzofuran-2-yl)-1,3-oxazole-5-carboxylate; Fura-2 AM

物化性质

• 密度: 1.4±0.1 g/cm3
• 沸点: 975.9±75.0 °C at 760 mmHg
• 分子式: C₄₄H₄₇N₃O₂₄
• 分子量: 1001.850
• 闪点: 544.0±37.1 °C
• 精确质量: 1001.254944
• PSA: 327.11000
• LogP: 2.90
• 蒸汽压: 0.0±0.3 mmHg at 25°C
• 折射率: 1.567
• 储存条件:

  密闭于-20 ºC阴凉干燥环境中

• 稳定性:

  遵照规定使用和储存则不会分解。

• 水溶解性: DMSO: soluble
• 分子结构:

  1、 摩尔折射率：233.52

  2、 摩尔体积（m³/mol）：715.1

  3、 等张比容（90.2K）：1960.2

  4、 表面张力（dyne/cm）：56.4

  5、 极化率（10 ^-24cm ³）：92.57

• 计算化学:

  1.疏水参数计算参考值（XlogP）:4.5

  2.氢键供体数量:0

  3.氢键受体数量:27

  4.可旋转化学键数量:37

  5.互变异构体数量:无

  6.拓扑分子极性表面积327

  7.重原子数量:71

  8.表面电荷:0

  9.复杂度:1780

  10.同位素原子数量:0

  11.确定原子立构中心数量:0

  12.不确定原子立构中心数量:0

  13.确定化学键立构中心数量:0

  14.不确定化学键立构中心数量:0

  15.共价键单元数量:1

• 更多:

  1. 性状：无可用

  2. 密度（g/mL,25/4℃）：无可用

  3. 相对蒸汽密度（g/mL,空气=1）：无可用

  4. 熔点（ºC）：无可用

  5. 沸点（ºC,常压）：无可用

  6. 沸点（ºC,5.2kPa）：无可用

  7. 折射率：无可用

  8. 闪点（ºC）：无可用

  9. 比旋光度（º）：无可用

  10. 自燃点或引燃温度（ºC）：无可用

  11. 蒸气压（kPa,25ºC）：无可用

  12. 饱和蒸气压（kPa,60ºC）：无可用

  13. 燃烧热（KJ/mol）：无可用

  14. 临界温度（ºC）：无可用

  15. 临界压力（KPa）：无可用

  16. 油水（辛醇/水）分配系数的对数值：无可用

  17. 爆炸上限（%,V/V）：无可用

  18. 爆炸下限（%,V/V）：无可用

  19. 溶解性：可溶于水

生物活性

• 描述: Fura-2 AM 是一种比率荧光 Ca2+ 指示剂。
• 相关类别:
  信号通路 >> 其他 >> 其他
  染料试剂
  研究领域 >> 其他
• 体外研究: 在装载750nM(n = 111),500nM(n = 119)或250nM(n = 130)Fura-2AM的tsA-201细胞中设想胰蛋白酶(100nM)介导的Ca2 +瞬变,并比较平均值。 Ca2 +在基线以上增加至使用1μMFura-2AM在初始实验中获得的值。对于750,500和250 nM,Ca2 +增加到基线以上是633.0±33.9 nM(n = 111,P> 0.05与1μMFura-2 AM相比),552.6±22.7 nM(n = 119,P> 0.05比较至1μMFura-2 AM)和616.0±24.9 nM(n = 130,P> 0.05与1μMFura-2 AM相比),分别为[1]。
• 细胞实验: 细胞负载Fura-2AM，其扩散穿过细胞膜并通过细胞酯酶去酯化以产生富含Fura-2的酸。将2mL培养基等分至15mL锥形管中，温热至37℃。加入2μLFura-2 AM原液，生成1μMFura-2 AM溶液。剧烈涡旋溶液1分钟。将加样溶液转移到35mm组织培养皿中，并将盖玻片与细胞一起转移到培养皿中。在黑暗的培养箱中将神经元在37度孵育30分钟。准备一个35毫米的培养皿，其中含有2毫升不含Fura-2 AM的组织培养基。从装载溶液中取出盖玻片并放入新盘中。将盖玻片安装在成像室[2]上。
• 参考文献:

  [1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24.

  [2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067.

MSDS

Section1. IDENTIFICATION OF THE SUBSTANCE/MIXTURE Product identifiers Product name: Fura 2-AM CAS-No.: 108964-32-5 Relevant identified uses of the substance or mixture and uses advised against Identified uses: Laboratory chemicals, Manufacture of substances Section2. HAZARDS IDENTIFICATION Classification of the substance or mixture Not a hazardous substance or mixture according to Regulation (EC) No 1272/2008 This substance is not classified as dangerous according to Directive 67/548/EEC. Label elements This substance is not classified as dangerous according to Directive 67/548/EEC. Other hazards - none Section3. COMPOSITION/INFORMATION ON INGREDIENTS Substances Synonyms: Fura-2 pentakis(acetoxymethyl) ester Formula: C44H47N3O24 Molecular Weight: 1.001,85 g/mol Section4. FIRST AID MEASURES Description of first aid measures If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. In case of skin contact Wash off with soap and plenty of water. In case of eye contact Flush eyes with water as a precaution. If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. Most important symptoms and effects, both acute and delayed To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. Indication of any immediate medical attention and special treatment needed no data available Section5. FIREFIGHTING MEASURES Extinguishing media Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Special hazards arising from the substance or mixture Carbon oxides, nitrogen oxides (NOx) Advice for firefighters Wear self contained breathing apparatus for fire fighting if necessary. Further information no data available Section6. ACCIDENTAL RELEASE MEASURES Personal precautions, protective equipment and emergency procedures Avoid dust formation. Avoid breathing vapors, mist or gas. Environmental precautions Do not let product enter drains. Methods and materials for containment and cleaning up Sweep up and shovel. Keep in suitable, closed containers for disposal. Reference to other sections For disposal see section 13. Section7. HANDLING AND STORAGE Precautions for safe handling Provide appropriate exhaust ventilation at places where dust is formed. Conditions for safe storage, including any incompatibilities Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: -20 °C Light sensitive. Store under inert gas. Specific end use(s) no data available Section8. EXPOSURE CONTROLS/PERSONAL PROTECTION Control parameters Components with workplace control parameters Exposure controls Appropriate engineering controls General industrial hygiene practice. Personal protective equipment Eye/face protection Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Skin protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Body Protection Choose body protection in relation to its type, to the concentration and amount of dangerous substances, and to the specific work-place., The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Respiratory protection Respiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Section9. PHYSICAL AND CHEMICAL PROPERTIES Information on basic physical and chemical properties a) AppearanceForm: crystalline Colour: yellow b) Odourno data available c) Odour Thresholdno data available d) pHno data available e) Melting point/freezingno data available point f) Initial boiling point and no data available boiling range g) Flash pointno data available h) Evaporation rateno data available i) Flammability (solid, gas) no data available j) Upper/lowerno data available flammability or explosive limits k) Vapour pressureno data available l) Vapour densityno data available m) Relative densityno data available n) Water solubilityno data available o) Partition coefficient: n- no data available octanol/water p) Auto-ignitionno data available temperature q) Decompositionno data available temperature r) Viscosityno data available s) Explosive propertiesno data available t) Oxidizing propertiesno data available Other safety information no data available Section10. STABILITY AND REACTIVITY Reactivity no data available Chemical stability no data available Possibility of hazardous reactions no data available Conditions to avoid Light. Incompatible materials Strong oxidizing agentsStrong oxidizing agents Hazardous decomposition products Other decomposition products - no data available Section11. TOXICOLOGICAL INFORMATION Information on toxicological effects Acute toxicity no data available Skin corrosion/irritation no data available Serious eye damage/eye irritation no data available Respiratory or skin sensitization no data available Germ cell mutagenicity no data available Carcinogenicity IARC:No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. Reproductive toxicity no data available Specific target organ toxicity - single exposure no data available Specific target organ toxicity - repeated exposure no data available Aspiration hazard no data available Potential health effects InhalationMay be harmful if inhaled. May cause respiratory tract irritation. IngestionMay be harmful if swallowed. SkinMay be harmful if absorbed through skin. May cause skin irritation. EyesMay cause eye irritation. Signs and Symptoms of Exposure To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. Additional Information RTECS: Not available Section12. ECOLOGICAL INFORMATION Toxicity no data available Persistence and degradability no data available Bioaccumulative potential no data available Mobility in soil no data available Results of PBT and vPvB assessment no data available Other adverse effects no data available Section13. DISPOSAL CONSIDERATIONS Waste treatment methods Product Offer surplus and non-recyclable solutions to a licensed disposal company. Contaminated packaging Dispose of as unused product. Section14. TRANSPORT INFORMATION UN number ADR/RID: -IMDG: -IATA: - UN proper shipping name ADR/RID: Not dangerous goods IMDG: Not dangerous goods IATA:Not dangerous goods Transport hazard class(es) ADR/RID: -IMDG: -IATA: - Packaging group ADR/RID: -IMDG: -IATA: - Environmental hazards ADR/RID: noIMDG Marine Pollutant: noIATA: no Special precautions for user no data available SECTION 15 - REGULATORY INFORMATION N/A SECTION 16 - ADDITIONAL INFORMATION N/A

毒性和生态

生态学数据: 该物质对环境可能有危害，对水体应给予特别注意。

安全

• 个人防护装备: Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
• 危险品运输编码: NONH for all modes of transport
• WGK德国: 3