Tetrahedron 2018-03-14

Synthesis of an electronically-tuned minimally interfering alkynyl photo-affinity label to measure small molecule–protein interactions

Chia-Fu Chang, Adelphe Mfuh, Jinxu Gao, Hung-Yi Wu, Christina M. Woo

Index: 10.1016/j.tet.2018.03.024

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Abstract

We report the synthesis of an electronically-tuned minimally interfering photo-affinity label (MI-PAL), a compact five-carbon tag functionalized with an alkyl diazirine and alkyne handle. MI-PAL is compatible with protein photo-conjugation, click chemistry and mass spectrometry and readily installed to complex molecules for biological target identification.