Room-temperature solid phase ionic liquid (RTSPIL) coated ω-transaminases: Development and application in organic solvents
B. Grabner, M.A. Nazario, M.T. Gundersen, S. Loïs, S. Fantini, S. Bartsch, J.M. Woodley, H. Gruber-Woelfler
Index: 10.1016/j.mcat.2018.03.012
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Abstract
ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co‐lyophilization, and showed increased stability in all of the five tested organic solvents. Co‐lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8‐fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5′‐phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5‐bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.