ChemBioChem 2018-03-30

Protein crystallography and site‐direct mutagenesis analysis of the poly(ethylene terephthalate) hydrolase PETase from Ideonella sakaiensis

Bing Liu; Lihui He; Liping Wang; Tao Li; Changcheng Li; Huayi Liu; Yunzi Luo; Rui Bao

Index: 10.1002/cbic.201800097

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Abstract

Compared with traditional recycle strategies, biodegradation provides a sustainable solution for poly (ethylene terephthalate) (PET) wastes disposal. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET, which provides a prominent prospect on PET degradation. Based on the biochemical analysis, we propose that the wide substrate‐binding pocket is critical for its excellent property on crystallized PET hydrolysis. Structure‐guided site‐directed mutagenesis exhibited improvement in PETase catalytic efficiency, providing valuable insight on how the molecular engineering of PETase can optimize its application in biocatalysis.