Synthesis of [1′, 2′, 5′, 2??13C4]??2′??Deoxy??D??adenosine by a Chemoenzymatic Strategy to Enable Labelling of Any of the 215 Carbon??13 and Nitrogen??15 …

N Ouwerkerk, J Boom, J Lugtenburg…

Index: Ouwerkerk, Niels; Van Boom, Jacques; Lugtenburg, Johan; Raap, Jan European Journal of Organic Chemistry, 2002 , # 14 p. 2356 - 2362

Full Text: HTML

Citation Number: 7

Abstract

Abstract Enzymatic trans-N-glycosylation has been selected as the method of choice by which to couple [13 C 1]-adenine to [13 C 3]-2-deoxy-D-ribose. The enzymatic pentosylation of the labelled adenine base was achieved in a two-step/one-pot reaction, starting from thymidine labelled in the sugar ring, but not at the thymine base. The efficiency of this thymidine phosphorylase catalysed (TP-catalysed) and purine nucleoside phosphorylase ...