A phosphoramidite approach using 2-cyanoethyl N, N-diisopropylchlorophosphoramidite was utilized for the first time to synthesize short chain cardiolipins. The approach was extended to synthesize long chain and their ether analogue. Optically active 1, 2-di-O-acyl- sn-glycerol or 1, 2-di-O-myristyl-sn-glycerol was coupled with phosphoramidite reagent and 2-benzyloxy-1, 3-propanediol in presence of 1H-tetrazole, followed by in situ oxidation, to ...