Determination and isolation of a thioesterase from passion fruit (Passiflora edulis Sims) that hydrolyzes volatile thioesters

…, I Cummins, D Brassington, R Edwards

Index: Tapp, Edward J.; Cummins, Ian; Brassington, David; Edwards, Robert Journal of Agricultural and Food Chemistry, 2008 , vol. 56, # 15 p. 6623 - 6630

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Citation Number: 5

Abstract

Volatile organosulfur compounds (VOSCs) are high impact aroma chemicals characteristic of tropical fruits which are active as both free thiols and the respective thioesters. Using a simple and sensitive colorimetric enzyme assay, a thioesterase activity toward VOSCs has been identified in ripening purple passion fruit (Passiflora edulis Sims). The assay was based on determining the release of free thiols from 2-methyl-3-furanthiol acetate using ...