Site??Specific Labeling of Proteins with a Chemically Stable, High??Affinity Tag for Protein Study

Y Yang, QF Li, C Cao, F Huang…

Index: Yang, Yin; Li, Qing-Feng; Cao, Chan; Huang, Feng; Su, Xun-Cheng Chemistry - A European Journal, 2013 , vol. 19, # 3 p. 1097 - 1103

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Citation Number: 23

Abstract

Abstract Site-specific labeling of proteins with paramagnetic lanthanides offers unique opportunities by virtue of NMR spectroscopy in structural biology. In particular, these paramagnetic data, generated by the anisotropic paramagnetism including pseudocontact shifts (PCS), residual dipolar couplings (RDC), and paramagnetic relaxation enhancement (PRE), are highly valuable in structure determination and mobility studies of proteins and ...

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