Abstract The present study deals with the synthesis of novel spiro [azetidine-2, 3′-indole]- 2′, 4 (1′ H)-dione derivative from the reactions of 3-(phenylimino)-1, 3-dihydro-2H-indol-2- one derivatives with chloracetyl chloride in the presence of triethylamine (TEA). All the compounds were characterized using IR, 1 H-NMR, MS, and elemental analysis. They were screened for their antibacterial and antifungal activities. The bacterial strains used were ...