Accurate measurements of 13C-13C J-couplings in the rhodopsin chromophore by double-quantum solid-state NMR spectroscopy

…, PHM Bovee-Geurts, OG Johannessen…

Index: Lai, Wai Cheu; McLean, Neville; Gansmueller, Axel; Verhoeven, Michiel A.; Antonioli, Gian Carlo; Carravetta, Marina; Duma, Luminita; Bovee-Geurts, Petra H. M.; Johannessen, Ole G.; De Groot, Huub J. M.; Lugtenburg, Johan; Emsley, Lyndon; Brown, Steven P.; Brown, Richard C. D.; DeGrip, Willem J.; Levitt, Malcolm H. Journal of the American Chemical Society, 2006 , vol. 128, # 12 p. 3878 - 3879

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Citation Number: 42

Abstract

A new double-quantum solid-state NMR pulse sequence is presented and used to measure one-bond 13C-13C J-couplings in a set of 13C2-labeled rhodopsin isotopomers. The measured J-couplings reveal a perturbation of the electronic structure at the terminus of the conjugated chain but show no evidence for protein-induced electronic perturbation near the C11-C12 isomerization site. This work establishes NMR methodology for measuring ...