New approach to immunochemical determinations for triclopyr and 3, 5, 6-trichloro-2-pyridinol by using a bifunctional hapten, and evaluation of polyclonal antiserum

E Watanabe, R Hoshino, Y Kanzaki…

Index: Watanabe, Eiki; Hoshino, Ryoko; Kanzaki, Yukiko; Tokumoto, Hiroshi; Kubo, Hiroaki; Nakazawa, Hiroyuki Journal of agricultural and food chemistry, 2002 , vol. 50, # 13 p. 3637 - 3646

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Citation Number: 12

Abstract

The present work describes the design and synthesis of the structurally unique hapten,“bifunctional hapten”, to produce a group-specific polyclonal antiserum to triclopyr and 3, 5, 6-trichloro-2-pyridinol. A bifunctional hapten was designed and synthesized by conjugating commercially available N ε-2, 4-dinitrophenyl (DNP)-l-lysine to triclopyr, and then coupling this to carrier proteins such as bovine serum albumin (BSA). The ...