Aminoacyl-tRNA synthesis by a resin-immobilized ribozyme

H Murakami, NJ Bonzagni, H Suga

Index: Murakami, Hiroshi; Bonzagni, Neil J.; Suga, Hiroaki Journal of the American Chemical Society, 2002 , vol. 124, # 24 p. 6834 - 6835

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Citation Number: 32

Abstract

We report herein a new method for the aminoacylation of tRNA, using a resin-immobilized ribozyme and the cyanomethyl ester (CME) of an amino acid substrate. The oxidized form of the ribozyme was immobilized on a hydrazine resin via covalent linkage. We performed aminoacylation of tRNAs using this ribozyme-resin to isolate aminoacyl-tRNAs. The column was recycled up to 5 times without significant activity loss. Thus, our ribozyme-based ...